First, use a wax pencil to draw a one on the microscopic lense slide to separate each type of bacteria that is walking to be sampled.

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If a basic stain of only one kind of bacteria, or solitary mixed sample the bacteria is gift prepared, no separation is required.
Article Summary: In bespeak to watch individual bacteria with a irradiate microscope, a bacter smear need to be attached come a slide and then stained. Here is the procedure.
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In stimulate to warmth fix a bacterial smear, that is crucial to an initial let the bacterial sample air dry. Then either ar the on slide in the slide holder of a microincinerator, or happen the dried slide through the fire of a Bunsen burner 3 or 4 times, smear side dealing with up. As soon as the slide is heat fixed, it have the right to then it is in stained.
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In bespeak to it is in able to clearly see individual bacteria, a sample that a bacterial colony must be mixed right into water or physiological saline. This help to evenly spread out out the bacter sample.

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Bacterial smear after heat fixing and before staining. Positive bacterial control in circle on the left, negative control ~ above right, and also unknown bacteria in facility circle. 
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