You are watching: Why do you gently heat the slide before staining
If a basic stain of only one kind of bacteria, or solitary mixed sample the bacteria is gift prepared, no separation is required.
Article Summary: In bespeak to watch individual bacteria with a irradiate microscope, a bacter smear need to be attached come a slide and then stained. Here is the procedure.
In stimulate to warmth fix a bacterial smear, that is crucial to an initial let the bacterial sample air dry. Then either ar the on slide in the slide holder of a microincinerator, or happen the dried slide through the fire of a Bunsen burner 3 or 4 times, smear side dealing with up. As soon as the slide is heat fixed, it have the right to then it is in stained.
In bespeak to it is in able to clearly see individual bacteria, a sample that a bacterial colony must be mixed right into water or physiological saline. This help to evenly spread out out the bacter sample.
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Bacterial smear after heat fixing and before staining. Positive bacterial control in circle on the left, negative control ~ above right, and also unknown bacteria in facility circle.
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